大理大学学报 ›› 2021, Vol. 6 ›› Issue (10): 28-.DOI: 10. 3969/j. issn. 2096-2266. 2021. 10. 005

• 基础医学 • 上一篇    下一篇

大学生肠道阴沟肠杆菌与体质指数的相关性研究

罗春雨,李亚飞,刘 奇   

  1. (大理大学基础医学院,云南大理 671000
  • 收稿日期:2021-05-19 修回日期:2021-07-11 出版日期:2021-10-15 发布日期:2021-12-11
  • 通讯作者: 刘奇,副教授,博士, E-mail:Qiliu@aliyun.com。
  • 作者简介:罗春雨,硕士研究生,主要从事免疫学研究。
  • 基金资助:
    大理大学创新团队项目(ZKLX2019105

Study on the Correlation between Enterobacter cloacae and Body Mass Index of College Students

Luo Chunyu Li Yafei Liu Qi   

  1. Pre-clinical CollegeDali University Dali Yunnan 671000China

  • Received:2021-05-19 Revised:2021-07-11 Online:2021-10-15 Published:2021-12-11

摘要:

目的:探讨阴沟肠杆菌在肠杆菌科中的菌落数比例及其与大学生体质指数(BMI)之间的关系。方法:对健康大学生粪便标本进行涂片及革兰氏染色;使用伊红美蓝培养基培养稀释后的粪便标本,并对其上的粉色菌落比例进行统计,通过阴沟肠杆菌的特异性引物进行菌落PCR鉴定;分析阴沟肠杆菌菌落比例与大学生BMI的相关性。结果:伊红美蓝培养基上的粉色菌落为阴沟肠杆菌,通过菌落PCR可见阴沟肠杆菌的特异性385 bp条带。大学生BMI与其肠道中阴沟肠杆菌菌落数比例经Spearman秩相关分析,相关系数rs=0.529P0.001)。结论:健康大学生肠道阴沟肠杆菌在肠杆菌科中的比例与BMI呈正相关。

关键词: font-family:宋体, ">大学生;肥胖;体质指数;肠道菌群;阴沟肠杆菌

Abstract:

ObjectiveTo investigate the colony ratio of Enterobacter cloacae in Enterobacteriaceae and its relationship with the body mass index BMI of college students. Methods Smear and Gram stain were performed on stool specimens of healthy college students the diluted stool specimens were cultured with eosin methylene blue medium and the proportion of pink colonies on it was counted. The specific primers of Enterobacter cloacae were performed colony PCR identification analysis of the correlation between the colony ratio of Enterobacter cloacae and the BMI of college students. Results The pink colony on the eosin methylene blue medium was Enterobacter cloacae and the specific 385 bp band of Enterobacter cloacae could be seen by colony PCR. The ratio of the BMI of college students to the number of Enterobacter cloacae colonies in their intestines was analyzed by Spearman's rank correlation and the correlation coefficient rs= 0.529 P0.001. Conclusion The proportion of Enterobacter cloacae in the Enterobacteriaceae of healthy college students is positively correlated with their BMI.

Key words:

"> college students">; obesity">; body mass index">; intestinal flora">; Enterobacter cloacae

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